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Preparation of [ 15 N]-CNCbl After extraction of cells and clean-up of the extracts from [ 15 N]-CDMG incubation as described in section “Extraction and Purification of Bacterial Cells,” the eluent was exposed to ambient light for 40 min before adding 30 μL of NaCN solution (1% in water, w/v)

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For instance, supplementing aged mice with NMN, which increases NAD+, improved oocyte quality and embryo blastocyst rates